Methods of Standardization of Antibiotics Methods of Standardization of Antibiotics Antibiotics have been a cornerstone in medical treatment, fighting bacterial infections and saving lives. To ensure their efficacy and safety, it is crucial to standardize their production. Here are some methods used for the standardization of antibiotics: 1. Potency Assay Potency assay is a common method to determine the strength of antibiotics. It involves measuring the antibiotic's ability to inhibit the growth of a specific strain of bacteria. By comparing the results with a reference standard, the potency of the antibiotic can be quantified. 2. Microbiological Assay In microbiological assay, the potency of antibiotics is evaluated by measuring the response of living organisms, typically bacteria, to the antibiotic. This method helps determine the concentration of the antibiotic required to inhibit microbial growth effectively. 3. High-Performance Liquid Chromatogr...